TECHNICAL RESOURCES

• Overview of Protein products
• Protocols and Manuals
• Brochures and Flyers
• Vector maps/accession numbers for
  pCold Vectors
• MCS Restriction Site and Sequence
  Information for pCold Vectors (.pdf)
• Chaperone Plasmid Set Vector Maps
  (select Figure Thumbnail)
• Summary Protocol for Refolding CA Kit
• Licensing form for pCold Vectors (.pdf)
• Chaperone plasmid sequence
  and modification request form (.pdf)
• Frequently Asked Questions
  – Refolding CA Kit (.pdf)
  – Chaperone Plasmid Set (.pdf)
  – pCold Vectors (.pdf)
  – SPP System

GENERAL INFORMATION

• Overview of Protein products
• Model of Protein Folding (see second figure thumbnail)
• Principle of the Refolding CA Kit (select figure thumbnail)
• Principle of SPP System (see first figure thumbnail)

PRODUCTS

• Recombinant Protein Folding & Expression
• Protein Sequencing & Analysis

ARTICLES

• Unfolding the Potential of Proteins.

REFERENCES

• Cycloamylose as an efficient artificial chaperone for protein refolding.  S. Machida, et al. (2000 Dec 8) FEBS Letters, Volume 486, Issue 2,  486(2): 131-5.
• Cold-shock induced high-yield protein production in Escherichia coli. Guoliang Qing, et al. (01 Jul 2004) Nature Biotechnology 22, 877-82.
• Single Protein Production in Living Cells Facilitated by an mRNA Interferase. Suzuki, M. et al. (2005) Molecular Cell, Volume 18, 253-161.
• Insights into the mRNA cleavage mechanism by MazF, an mRNA interferase. Zhang, Y. et al. (2004) Journal of Biological Chemistry, Volume 280, 3143-3150.
• MazF Cleaves Cellular mRNAs Specifically at ACA to Block Protein Synthesis in Escherichia coli. Zhang, Y. et al. (2003) Molecular Cell, Volume 12, 913-923.

APPLICATION DATA

• Examples for pCold
• Examples for SPP System™ (Single Protein Production System)